Related Experiment Videos

Interleukin-6 and retroperitoneal fibromatosis from SRV-2-infected macaques with simian AIDS

S T Roodman1, M D Woon, J W Hoffmann

  • 1Department of Pathology, St. Louis University School of Medicine, MO 63104.

Journal of Medical Primatology
|June 1, 1991
PubMed

Insights

Interleukin-6 (IL-6) acts as an autocrine growth factor in macaques with simian retrovirus type 2 (SRV-2) infection. SRV-2 increases IL-6 production in retroperitoneal fibromatosis cells, promoting tumor growth.

Area of Science:

  • Virology
  • Immunology
  • Oncology

Background:

  • Simian retrovirus type 2 (SRV-2) infection in macaques is associated with retroperitoneal fibromatosis (RF).
  • The role of Interleukin-6 (IL-6) in the pathogenesis of SRV-2-induced RF is not fully understood.

Purpose of the Study:

  • To investigate the role of IL-6 as an autocrine growth factor for RF cells in SRV-2 infected macaques.
  • To determine the effect of SRV-2 on IL-6 production in RF cells.

Main Methods:

  • Quantification of IL-6 levels in serum, ascites, and cell culture media.
  • Measurement of IL-6 mRNA and protein expression in RF cells.
  • Incubation of RF cells with exogenous SRV-2.

Main Results:

  • Elevated IL-6 levels were detected in serum and ascites of SRV-2 infected macaques with RF.
  • IL-6 mRNA levels increased significantly in RF cells exposed to SRV-2.
  • SRV-2 was shown to enhance both IL-6 mRNA and protein production in RF cells.

Conclusions:

  • Interleukin-6 (IL-6) functions as an autocrine growth factor for retroperitoneal fibromatosis (RF) cells in macaques infected with simian retrovirus type 2 (SRV-2).
  • SRV-2 infection stimulates IL-6 production, contributing to the development and progression of RF.

Related Concept Videos