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Updated: Aug 9, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
RNA-dependent RNA polymerases of plants
1Virus Laboratory and Department of Molecular Biology, University of California, Berkeley, California 94720.
Abstract:
The existence of RNA-dependent RNA polymerases (EC 2.7.7.48) in plants has been definitely proven by their isolation in pure form from cucumber and tobacco in our laboratory and from cowpea at Wageningen. These enzymes are single-chain proteins of 100-130 kilodaltons. They show clear physical and biochemical differences characteristic for a given plant species, even when their amounts in the plants were greatly increased prior to isolation by infection with the same virus. The role of these enzymes in plant physiology remains unknown.
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