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Determination of infectious bursal disease virus titration and neutralization endpoints using fluorogenic staining
G R Bayyari1, J K Skeeles, J D Story
1Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
Abstract:
An automated method for determining infectious bursal disease virus (IBDV) titration and neutralization endpoints is described. The method employs the fluorogenic ester carboxyfluorescein-diacetate (CFDA) to stain cell monolayers in 96-well plates and a fluorescence-concentration analyzer. Titration results are compared with immunofluorescence and plaque assay titers. Virus-neutralization endpoint determination is objective, and the endpoints of replicate tests were equivalent or within one dilution of variability. Tests can be automatically screened as any percentage of a positive control or any standard deviation from a negative control.