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Updated: Aug 9, 2026

Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus
Published on: December 22, 2023
Hairy roots are more sensitive to auxin than normal roots
1Laboratoire de Physiologie Cellulaire Végétale, Centre National de la Recherche Scientifique/Institut National de la Recherche Agronomique, avenue de la Terrasse, 91190 Gif sur Yvette, France.
Abstract:
Responses to auxin of Lotus corniculatus root tips or protoplasts transformed by Agrobacterium rhizogenes strains 15834 and 8196 were compared to those of their normal counterparts. Three different types of experiments were performed, involving long-term, medium-term, or short-term responses to a synthetic auxin, 1-naphthaleneacetic acid. Root tip elongation, proton excretion by root tips, and transmembrane electrical potential difference of root protoplasts were measured as a function of exogenous auxin concentration. The sensitivity of hairy root tips or protoplasts to exogenous auxin was found to be 100-1000 times higher than that of untransformed material.
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