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Updated: Aug 9, 2026

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Published on: December 30, 2014
[Functional expression of adenylyl cyclase and phosphodiesterase in ejaculated human spermatozoa]
Zhi-ming Cai1, Yao-ting Gui, Lian-dian Guo
1The Center for Reproductive Medicine, Peking University Shenzhen Hospital, Guangdong, China.
Objective:
To compare the differences of expressions of adenylyl cyclase (AC) and phosphodiesterase (PDE) in ejaculated spermatozoa between healthy volunteers and the patients with asthenospermia.
Methods:
Ejaculated spermatozoa were collected from healthy volunteers and the patients with asthenospermia. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect mRNA expression of AC and PDE subtypes in human spermatozoa. The concentrations of cAMP and cGMP in the samples were detected by enzyme-linked immunosorbent assay (ELISA).
Results:
Compared with healthy volunteers, expression of sAC mRNA and concentration of cAMP were significantly decreased in the patients with asthenospermia (P < 0.01) , while the expression of PDE4C mRNA was significantly increased at the same time (P <0.01). There were no marked differences in the expression of ACIII mRNA and concentration of cGMP between the two groups.
Conclusion:
The sAC down-regulation and PDE4C up-regulation are possible reasons for asthenospermia.
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