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Updated: Aug 9, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
Published on: March 18, 2017
Higher affinity quadruply hydrogen-bonded complexation with 7-deazaguanine urea
Hugo C Ong1, Steven C Zimmerman
1Department of Chemistry, 600 South Mathews Avenue, University of Illinois, Urbana, Illinios 61801, USA.
Abstract:
[structure: see text] UG forms a highly stable quadruply hydrogen-bonded heterocomplex with DAN, but the fidelity of the complex is lowered somewhat by the Hoogsteen-side oligomerization of UG (K(assoc) approximately 230 M(-)(1), CDCl(3)). DeUG was prepared as a more robust analogue of UG lacking the Hoogsteen nitrogen atom. Remarkably, the deaza analogue, DeUG, forms a much more stable complex with DAN (>10-fold higher K(assoc) for DeUG.DAN vs UG.DAN) but also dimerizes more strongly (K(dim) = 880 +/- 40 M(-)(1), CDCl(3)) by adopting a conformation preorganized for both binding and dimerization.
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