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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
Published on: June 28, 2018
Enhancer/promoter activities of regulatory regions of representative JC virus isolates
1Mercer University School of Medicine, Macon, Georgia.
Archives of Virology
|January 1, 1991
Abstract:
The enhancer/promoter activities of JC virus isolates MAD1, MAD8, and MAD11 in HeLa cells and in human glial cells expressing either SV40 or JCV(MAD1) tumor antigens were significantly different from one another in each cell line.
Insights
JC virus enhancer/promoter activities varied significantly between isolates MAD1, MAD8, and MAD11. These differences were observed in both HeLa and human glial cells expressing tumor antigens.
Area of Science:
- Virology
- Molecular Biology
- Oncology
Background:
- The JC virus (JCV) is a human polyomavirus associated with progressive multifocal leukoencephalopathy (PML).
- Tumor antigens, such as Simian virus 40 (SV40) and JCV(MAD1) tumor antigens, play crucial roles in viral replication and oncogenesis.
- Understanding the regulatory elements of JCV, like enhancer/promoter regions, is vital for comprehending viral pathogenesis.
Purpose of the Study:
- To investigate and compare the enhancer/promoter activities of different JC virus isolates.
- To analyze the differential expression of JCV isolates in various human cell lines.
- To determine the influence of tumor antigens on JCV enhancer/promoter function.
Main Methods:
- Utilized reporter gene assays to measure enhancer/promoter activity.
- Employed HeLa cells and human glial cells for in vitro studies.
- Expressed specific tumor antigens (SV40 and JCV(MAD1)) within the cell lines to mimic infection or oncogenic conditions.
Main Results:
- Significant differences in enhancer/promoter activities were observed among JCV isolates MAD1, MAD8, and MAD11.
- Differential activities were consistent across both HeLa and human glial cell lines.
- The presence of SV40 or JCV(MAD1) tumor antigens modulated the enhancer/promoter activities of the JCV isolates.
Conclusions:
- JC virus isolates exhibit distinct enhancer/promoter activities.
- Cell type and the expression of specific tumor antigens significantly influence JCV enhancer/promoter function.
- These findings contribute to understanding the molecular mechanisms underlying JCV-associated diseases.
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