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Updated: Aug 9, 2026
Method of Studying Palatal Fusion using Static Organ Culture
Published on: September 19, 2015
[Establishment and application of fetal rolling palate organ culture model of mouse]
Jing Han1, Ying Xiao, Jiu-Xiang Lin
1Department of Food Science and Nutrition, School of Public Health, Peking University, Beijing 100083, China.
Objective:
To investigate the effect of all-trans retinoic acid (atRA) on the development of mouse palates and its possible mechanism, a model of fetal mouse rolling plate organ culture was establishted.
Methods:
Mouse embryonic palates were explanted on GD12 and cultured in a roller device for 72h induced by atRA in different concentrations from 10(-5) micromol/L to 10(-1) micromol/L.
Results:
Similar to development in vivo, palates in control normal fused. Compared with the control, palates development and fusion were promoted in group of 10(-15) micromol/L, while were inhibited in groups of 10(-4) micromol/L or greater, resulting in cleft palate. The proportion of fusion was reduced in a significant dose-response pattern.
Conclusion:
In the model, the cultured palates continued developing and fusing. Palatal fusion was promoted by normally atRA in 10(-5) micromol/L level and were inhibited by atRA in 10(-4) micromol/L level or greater, which resulting in cleft palate. These mentioned confirm the validity and reliability of this model.
