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Updated: Aug 8, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Identification of Aeromonas culicicola by 16S rDNA RFLP
Adam Kaznowski1, Edyta Konecka
1Department of Microbiology, Institute of Experimental Biology, A Mickiewicz University, Poznań, Poland. akazn@amu.edu.pl
Abstract:
Studies were conducted on the improvement of A. culicicola identification. This species is phenotypically very similar to A. veronii biotype sobria, A. sobria, and A. allosaccharophila. The sequences of 16S rDNA of A. culicicola isolates show the highest similarity with A. jandaei, A. veronii, and A. caviae. Digestion of 16S rDNA PCR product with AluI and MboI restriction endonucleases allowed discriminating A. culicicola from all other Aeromonas species with the exception of A. jandaei. Additional digestion of 16S rDNA PCR product with BceAI showed a possibility of distinguishing A. jandaei from A. culicicola.
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