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Updated: Aug 9, 2026

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
Published on: June 25, 2015
Co-expression of interleukin-2 by a bicistronic plasmid increases the efficacy of DNA immunization to prevent
Andreas Henke1, Nadine Rohland, Roland Zell
1Institute of Virology and Antiviral Therapy, Medical Center, Friedrich Schiller University, Jena, Germany. i6hean@rz.uni-jena.de
Abstract:
A promising approach to protect susceptible individuals against severe diseases is the inoculation of plasmids. Such DNA vaccines against influenza virus infections were quite efficient in different animal models; but still this procedure is not in clinical use until today. The present study reports the generation and characterization of bicistronic plasmids which enables the expression of influenza A virus gene sequences together with immunostimulatory cytokines demonstrating that among these cytokines especially interleukin-2 (IL-2) was efficient to prevent a lethal influenza virus infection in mice.
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