Related Experiment Video
Updated: Aug 9, 2026
10:14
An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
Published on: November 1, 2018
Differential nephron HO-1 expression following glomerular epithelial cell injury
Prasun K Datta1, Sreenivas Reddy, Mukut Sharma
1Laboratory of AIDS Pathogenesis and Molecular Therapeutics, Department of Neuroscience, Center for Neurovirology, Temple University School of Medicine, Philadelphia, PA 19122, USA. dattapk@temple.edu
Nephron. Experimental Nephrology
|April 8, 2006
Summary
Proteinuria upregulates heme-oxygenase-1 (HO-1) in kidney tubules, a process influenced by filtered inducers and free radicals. Scavenging free radicals enhances HO-1 expression, revealing segment-specific responses in the nephron.
Area of Science:
- Nephrology
- Biochemistry
- Molecular Biology
Background:
- Proteinuria involves increased heme-oxygenase (HO) in the nephron.
- Heme-oxygenase-1 (HO-1) is the inducible isoform of HO.
- Puromycin aminonucleoside (PAN) induces proteinuria by disrupting the glomerular barrier.
Purpose of the Study:
- To investigate the nephron distribution of HO-1 expression in a PAN-induced proteinuria model.
- To determine the role of free radicals in modulating HO-1 expression during proteinuria.
Main Methods:
- Rats were treated with PAN, a free radical scavenger (PBN), or both.
- Urine and kidney tissue were analyzed for protein, nitric oxide, malonyldialdehyde, and HO-1 expression.
- Immunohistochemistry and Western blot were used to assess HO-1 levels.
Main Results:
- PAN induced proteinuria and increased malonyldialdehyde excretion.
- HO-1 was upregulated in tubules and glomerular parietal cells post-PAN treatment.
- PBN administration reduced proteinuria and enhanced tubular HO-1 expression.
Conclusions:
- Nephron HO-1 upregulation occurs downstream of the glomerular barrier, mediated by filtered inducers.
- Free radical scavenging potentiates HO-1 induction and highlights segment-specific nephron responses.
Related Concept Videos
01:10Nephrons
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma happens...
01:29Acute Kidney Injury II: Pathophysiology
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
01:28Glomerular Filtration Rate and its Regulation
The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
01:20Renal Corpuscle
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...
01:28Diabetic Nephropathy
Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...
01:22Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion
The kidneys maintain homeostasis through filtration, reabsorption, and secretion. Tubular reabsorption and secretion are crucial in forming urine and regulating electrolytes, water balance, and waste elimination.Tubular Reabsorption and Secretion ProcessesTubular reabsorption is the process that reclaims essential substances such as electrolytes, glucose, amino acids, and water from the glomerular filtrate back into the bloodstream. This is achieved through passive and active transport...