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The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
Expression and function profiling of orphan nuclear receptors using bacterial artificial chromosome (BAC)
Eric Nemoz-Gaillard1, Ming-Jer Tsai, Sophia Y Tsai
1Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
Abstract:
The long term goal of the Nuclear Receptor Signaling Atlas (NURSA) resides in unraveling the physiological and pathological functions of nuclear receptors (NRs) at the molecular, biochemical and cellular levels. This multi-oriented task requires complementary approaches in order to determine the specific function(s) and precise expression and receptor activity patterns for each individual conventional or orphan receptor. To attain this objective, we have chose to turn to technologies recently made available to engineer bacterial artificial chromosomes (BACs).
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