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Transgenic mouse for conditional, tissue-specific Cox-2 overexpression.

Ken-ichiro Kamei1, Tomo-o Ishikawa, Harvey R Herschman

  • 1Department of Molecular and Medical Pharmacology, Molecular Biology Institute, Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA.

Genesis (New York, N.Y. : 2000)
|April 11, 2006
PubMed
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Researchers developed a novel cyclooxygenase-2 (Cox-2) conditional overexpression transgenic mouse model. This Cox-2 COE mouse allows for noninvasive monitoring of Cox-2 expression and facilitates studies in various diseases.

Area of Science:

  • Biomedical Science
  • Genetics
  • Molecular Biology

Background:

  • Cyclooxygenase-2 (Cox-2) plays a critical role in inflammation and disease.
  • Understanding Cox-2 overexpression is crucial for developing targeted therapies.
  • Existing models may not fully capture the dynamic expression of Cox-2.

Purpose of the Study:

  • To create a conditional overexpression transgenic mouse model for cyclooxygenase-2 (Cox-2).
  • To enable noninvasive, in vivo monitoring of Cox-2 expression.
  • To provide a valuable tool for studying the role of Cox-2 in various pathological conditions.

Main Methods:

  • Construction of a Cox-2 conditional overexpression (COE) transgenic mouse.
  • Incorporation of a CAG promoter, Cox-2, humanized Renilla luciferase (hRL), and a Cre-excisable STOP sequence.

Related Experiment Videos

  • Utilized adenovirus carrying Cre recombinase (Ad.CMV.Cre) for conditional gene induction and in vivo imaging.

    • Main Results:

    • Successful generation of Cox-2 COE mice with Cre-inducible Cox-2 and hRL expression.
    • Demonstrated elevated hepatic Cox-2 expression and hRL signal upon Ad.CMV.Cre administration.
    • Confirmed Cox-2 and hRL expression and increased PGE(2) production in infected Cox-2 COE embryonic fibroblasts.

    Conclusions:

    • The developed Cox-2 COE mouse model enables precise, inducible, and monitorable Cox-2 overexpression.
    • This model is suitable for investigating Cox-2's role in cardiovascular disease, inflammation, neurodegeneration, and cancer.
    • The noninvasive imaging capability offers advantages for longitudinal studies.