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Updated: Aug 9, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
Published on: September 2, 2020
Semipreparative-scale gas-chromatographic separation of filbertone enantiomers
Gracia Patricia Blanch1, Marisa L Ruiz del Castillo
1Instituto de Fermentaciones Industriales, Consejo Superior Investigaciones Cientificas (CSIC), c/ Juan de la Cierva 3, Madrid, Spain. gblanch@ifi.csic.es
Abstract:
The enantioselectivity of heptakis(2,3-di-O-acetyl-6-O-tert-butyldimethylsilyl-beta-CD) toward racemic filbertone (E-5-methyl-hep-2-en-4-one) was studied by performing the chiral separation on a capillary column, a thick-film wide-bore column and a semipreparative column. The semipreparative enantioseparation of filbertone was achieved at 80 degrees C by using a packed column providing (R)- and (S)-enantiomers of filbertone with ee 90 and 96%, respectively. The isolated enantiomers (approximately 250 microg each, ee = 90-96%) may be used for studies on the relationship of chirality and biological activity by olfactory screening and toxicological studies.
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