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Low Ca2+ plus tetraethylammonium medium: its usefulness in studying the effect of Ca antagonist on adrenergic
1Department of Pharmacology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Japanese Journal of Pharmacology
|August 1, 1991
Abstract:
The low Ca2+ + tetraethylammonium (TEA) medium enhanced electrically evoked norepinephrine release by 3.5-fold in canine saphenous veins. omega-Conotoxin GVIA (omega-CTX) inhibited the neurotransmission more markedly in low Ca2+ + TEA medium than in normal Krebs medium. This is the case for the inhibition by tetrodotoxin, though to a lesser extent. The inhibition by omega-CTX was competitively antagonized by elevating external Ca2+. The results indicate that low Ca2+ + TEA medium is useful for studying the effect of Ca antagonists on adrenergic neurotransmission, because omega-CTX acts more effectively in this medium.