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Updated: Aug 9, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Cardiolipin in hydrogenosomes: evidence of symbiotic origin
Ivone de Andrade Rosa1, Marcelo Einicker-Lamas, Róbson Roney Bernardo
1Laboratorio de Ultraestrutura Celular, Univeridade Santa Ursula, Rio de Janeiro, Brazil.
Abstract:
Hydrogenosomes are found in organisms that lack typical mitochondria. Cardiolipin is a phospholipid located exclusively in bacterial membranes and the inner membrane of mitochondria. Here we show, by cell fractionation, thin-layer chromatography, high-pressure liquid chromatography, and matrix-assisted laser desorption ionization-time of flight mass spectrometry that hydrogenosomes of Tritrichomonas foetus, a cattle vaginal parasite, contain cardiolipin, which is strong evidence for its endosymbiotic origin.
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