Related Experiment Video
Updated: Aug 9, 2026

06:07
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Published on: August 5, 2022
A lag-phase in the reduction of flavin dependent thymidylate synthase (FDTS) revealed a mechanistic missing link
Aaron Mason1, Nitish Agrawal, M Todd Washington
1Department of Chemistry, University of Iowa, Iowa City, IA, USA. amnon-kohen@uiowa.edu
Abstract:
An unexpected substrate-dependent lag-phase, found in the single turnover reduction of FDTS bound flavin, sheds light on the molecular mechanism of this alternative thymidylate synthase.
