Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simultaneous analysis of cationic, anionic, and neutral compounds using monolithic CEC columns.

Masaru Kato1, Yuko Onda, Kumiko Sakai-Kato

  • 1Department of Analytical Chemistry, School of Pharmaceutical Sciences and COE Program in the 21st Century, University of Shizuoka, 52-1 Yada Suruga-ku, Shizuoka, 422-8526, Japan.

Analytical and Bioanalytical Chemistry
|April 13, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New-onset Systemic Lupus Erythematosus after Methimazole Exposure Followed by Autoantibody Emergence.

Internal medicine (Tokyo, Japan)·2026
Same author

Low-dose, not low-risk: potential adrenal suppression identified by a risk-based approach to ACTH testing.

Clinical rheumatology·2026
Same author

JCS/JPCPHS 2025 Guideline on Pulmonary Hypertension and Pulmonary Embolism/Deep Vein Thrombosis.

Circulation journal : official journal of the Japanese Circulation Society·2026
Same author

Deletion of Tgf-β1 From CD206<sup>+</sup> M2 Macrophages Ameliorates Obesity-Induced Suppression of Myogenesis and AMPK Phosphorylation in Skeletal Muscle.

Journal of cachexia, sarcopenia and muscle·2026
Same author

Lansoprazole-Induced Microscopic Colitis Mimicking Anticancer Drug-Related Diarrhea During Lenvatinib and Pembrolizumab Combination Therapy: A Case Report.

Clinical case reports·2026
Same author

A Rare Case of Bartter Syndrome Type 3 Diagnosed in Elderly Age.

Case reports in endocrinology·2026

A novel capillary electrochromatography column enables simultaneous analysis of charged compounds. Optimized poly(ethylene glycol) content and sheath liquid ensure stable, rapid separation and detection of diverse analytes.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrochromatography (CEC) offers powerful separation capabilities.
  • Simultaneous analysis of compounds with varying charges presents a challenge.

Purpose of the Study:

  • To develop and characterize a new CEC column for simultaneous separation of cationic, neutral, and anionic compounds.
  • To optimize separation conditions for enhanced analytical performance using CEC coupled with electrospray ionization mass spectrometry (CEC-ESI-MS).

Main Methods:

  • Three silica monolith columns with varying poly(ethylene glycol) (PEG) content were synthesized and evaluated.
  • Optimized separation programs and sheath liquid compositions were determined.
  • CEC-ESI-MS was employed for sensitive detection of analytes.

Related Experiment Videos

Main Results:

  • A column with 80 mg PEG content achieved separation of diverse analytes within 15 minutes using 1 M formic acid.
  • Analyte migration order (cationic, neutral, anionic) was primarily governed by electrophoresis.
  • Stable analysis was facilitated by hydrodynamic flow, and a sheath liquid of 1% acetic acid in 50% methanol enabled detection of all tested analytes.

Conclusions:

  • The developed PEG-modified silica monolith column is effective for simultaneous CEC-ESI-MS analysis of mixed-charge compounds.
  • Electrophoretic migration and hydrodynamic flow are key factors for successful separation and stable analysis.
  • The optimized method demonstrates broad applicability for analyzing various small molecules, including amino acids and metabolites.