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[Purification of total flavones from Morus alba L.by macroporous adsorbents and kinetic model for the process]
Jing-jing Chen1, Xiang-rong Li, Xiao Fang
1College of Pharmaceutical Science, Zhejiang University, Hangzhou 310031, China.
Objective:
To establish the separation and purification technology for the total flavones from Morus alba L.(MTF), and present a kinetic model for this process.
Methods:
Three types of macroporous adsorbents were tested to separate and purify MTF, and to evaluated by adsorption capacity, elution ratio and product purity. The curves of dynamic adsorption-elution process was plotted and the kinetic equations were presented.
Result:
Among three types of macroporous adsorbents, the NKA-9 type showed better property with the adsorption capacity of 43.4 mg.g(-1)and the elution ratio of 98.2%. The purity of MTF product reached to 58.2 % in the technological conditions following: extract sample concentration of 20 mg.ml(-1), 6 times washing water and elution reagent of 70% ethanol. The adsorbent could be used for 4 times repeatedly. The kinetic equation was y=u(1 - e(-kx)).
Conclusion:
The NKA-9 type of macroporous absorbent is suitable to purify the MTF.
