Related Experiment Video
Updated: Aug 9, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Perturbation-correlation moving-window two-dimensional correlation spectroscopy
Shigeaki Morita1, Hideyuki Shinzawa, Isao Noda
1Department of Chemistry, School of Science and Technology, Kwansei-Gakuin University, Sanda 669-1337, Japan.
Abstract:
A new method of analysis, perturbation-correlation moving-window two-dimensional (PCMW2D) correlation spectroscopy, is proposed. For a spectral data set collected under an external perturbation, this method provides a pair of synchronous and asynchronous two-dimensional correlation spectra plotted on a plane between a spectral variable (e.g., wavenumber) axis and a perturbation variable (e.g., temperature) axis. One of the advantages of this new correlation analysis method is that complicated spectral variation along the perturbation direction can be monitored. It has been found that the synchronous and asynchronous PCMW2D correlation spectra are similar to the first perturbation derivative and negative second perturbation derivative spectra of the original data, respectively. To demonstrate the potential of PCMW2D correlation spectroscopy, it has been applied to temperature-dependent infrared (IR) spectra of a poly(vinyl alcohol) (PVA) film. The thermal behavior of the PVA film has been revealed by the PCMW2D correlation analysis. Two characteristic cross-peaks are observed in the synchronous PCMW2D correlation spectra generated from the temperature-dependent IR spectra between the crystalline phase C-O stretching band at 1141 cm-1 and the melting temperature of 209 degrees C and between the amorphous phase C-O stretching band at 1095 cm-1 and another specific temperature of 233 degrees C. This specific temperature of 233 degrees C corresponds to the thermal degradation temperature due to the elimination of the hydroxyl group attached to the main chain.
More Related Videos
Related Concept Videos
2D NMR: Overview of Heteronuclear Correlation Techniques
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
2D NMR: Homonuclear Correlation Spectroscopy (COSY)
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration
According to Hooke's law, the vibrational frequency is directly proportional to the...
IR Spectroscopy: Molecular Vibration Overview
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

