Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An antibody-based microarray assay for small RNA detection.

Zonglin Hu1, Aixia Zhang, Gisela Storz

  • 1Bacterial Toxins and Therapeutics Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Nucleic Acids Research
|April 15, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Expanded Biopsychosocial Model Elucidates the Association Between Pre-Pregnancy Overweight/Obesity and Delayed Lactogenesis II: A Prospective Cohort Study.

Maternal & child nutrition·2026
Same author

Distinct mechanisms of dry and wet ball milling on foxtail millet starch: From physical agglomeration to molecular deconstruction.

Food chemistry·2026
Same author

Distribution of prophage-encoded Pas sRNAs across pathogenic <i>Escherichia coli</i>.

bioRxiv : the preprint server for biology·2026
Same author

Inhibitory Effect of Music Therapy Combined with Comfort Care on Reflux Events during Enteral Nutrition in Comatose Patients.

Noise & health·2026
Same author

The Relationship Between Midwives' Psychological Capital and Core Competency: A Latent Profile Analysis.

International journal of women's health·2026
Same author

Childhood adversity and multi-dimensional cognitive dysfunction in major depressive disorder: investigating heterogeneous effects across gender and educational in the UK Biobank.

BMC psychology·2026
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

This study introduces a novel antibody-based method for detecting RNA on microarrays. This technique efficiently detects small and structured RNAs, overcoming limitations of current methods for gene expression analysis.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • RNA detection on microarrays is crucial for molecular biologists.
  • Existing methods often fail to detect structured and small RNA species effectively.
  • There is a need for improved RNA detection techniques on microarrays.

Purpose of the Study:

  • To develop a novel, antibody-based method for direct RNA detection on DNA microarrays.
  • To overcome the limitations of current methods in detecting structured and small RNAs.
  • To enable efficient and quantitative analysis of gene expression.

Main Methods:

  • Direct hybridization of unmodified RNA to DNA microarrays.
  • Detection using the S9.6 mouse monoclonal antibody, specific for DNA/RNA hybrids.

Related Experiment Videos

  • Signal amplification via fluorescently-labeled secondary antibodies or streptavidin.

    • Main Results:

    • The antibody-based method demonstrated superior efficiency in detecting low-abundance small RNAs from Escherichia coli compared to cDNA-based methods.
    • Detection of specific small RNA was achieved at concentrations as low as 10 pM (0.25 fmol).
    • The method proved robust, inexpensive, and capable of quantitative gene expression analysis.

    Conclusions:

    • The S9.6 antibody-based approach provides an efficient, robust, and cost-effective solution for RNA detection on microarrays.
    • This method overcomes previous limitations, enabling the detection of short and structured RNA species.
    • It offers a valuable tool for quantitative gene expression analysis in molecular biology.