Presence of unintended Agrobacterium tumefaciens cloning vector sequences in genetically modified plants
Katarina Björklöf1, Michael Färdig, Kirsten S Jørgensen
1Finnish Environment Institute, Research Department, Hakuninmaantie 6, FI-00430, Helsinki, Finland. katarina.bjorklof@ymparisto.fi
Abstract:
Agrobacterium transformation was used in the production of genetically modified plants from oilseed rape (Brassica napus) and tobacco (Nicotiana tabacum). After inoculation stop with the antibiotic timentin, a subsequent one-week treatment eliminated the vector bacterium from the oilseed rape plate explant cultures. From the tobacco, however, we recorded vector-derived signals one week after potting the regenerants in the greenhouse and still 10 weeks later. Genetically modified plants produced through Agrobacterium-transformation therefore cannot be guaranteed to be completely free of unintended vector sequences after antibiotic treatment.
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