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Published on: July 25, 2017
Cloning and production of a novel bacteriocin, lactococcin K, from Lactococcus lactis subsp. lactis MY23
Yun Seog Kim1, Min Ju Kim, Pil Kim
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Guseong-dong, 373-1, Yusong-gu, Daejeon, 305-701, Korea.
Abstract:
A gene encoding the antimicrobial peptide, lactococcin K, was isolated from Lactococcus lactis subsp. lactis MY23 then cloned and expressed in Escherichia coli. Because the expressed lactococcin K was formed as an inclusion body in recombinant E. coli, a fusion protein containing lactococcin K and maltose-binding protein (MBP) was produced in a soluble form. For high-level production of lactococcin K, we performed a pH-stat fed-batch culture to produce 43,000 AU lactococcin K ml(-1) in 12 h.
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