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Published on: May 11, 2017
Aluminium chloride enhances colchicine production in root cultures of Gloriosa superba
Seemanti Ghosh1, Biswajit Ghosh, Sumita Jha
1Department of Botany, Centre of Advanced Study in Cell and Chromosome Research, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India.
Abstract:
Root cultures of Gloriosa superba were treated with 5 mM: methyl jasmonate and 125 microM: AlCl3 which enhanced the intracellular colchicine content of the roots by 50-fold and 63-fold, respectively. Ten millimolar of CaCl2 and 1 mM: CdCl2 enhanced biomass significantly (7- to 8.6-fold, respectively) while maximum release of colchicine into the medium was obtained with 10 mM: CdCl2. Casein hydrolysate, yeast extract and silver nitrate had no significant effect on growth and colchicine accumulation in root cultures.

