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Updated: Aug 9, 2026
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
Published on: July 10, 2019
Direct force measurement of the interaction between liposome and the C2A domain of synaptotagmin I using atomic force
Jong-Ho Park1, Eun-Young Kwon, Hyo-Il Jung
1Department of Mechanical Engineering, Yonsei University, Seoul, South Korea.
Abstract:
The binding force between a liposome and the C2A domain of synaptotagmin I was determined by an atomic force microscopy (AFM). Liposomes were immobilized on the surface of the L1 sensor chip and the C2A domains, which recognize phosphatidylserine, were chemically conjugated onto a gold-coated cantilever tip. The average interaction force between the C2A domain and the liposome was 306 (+/-57) pN while the force between untreated cantilever and the liposome was 58 (+/-16) pN. This work helps understand the physicochemical interactions between proteins and lipid vesicles for the design of high affinity protein probes against the apoptotic cell surface.
