Automated on-line renewable solid-phase extraction-liquid chromatography exploiting multisyringe flow injection-bead...

Area of Science:

• Analytical Chemistry
• Separation Science
• Microfluidics

Background:

• Traditional on-line SPE methods often suffer from band broadening due to mobile phase incompatibility.
• Developing efficient and cost-effective sample preparation techniques is crucial for analyzing complex matrices.

Purpose of the Study:

• To propose and validate a novel lab-on-valve (LOV) system for on-line solid-phase extraction (SPE) coupled with high-performance liquid chromatography (HPLC).
• To assess the system's performance for the determination of pharmaceutical residues in environmental and biological samples.

Main Methods:

• The study utilized a multisyringe flow injection analysis (MSFIA) system integrated with a lab-on-valve (LOV) approach employing bead injection (BI).
• This hyphenated system performed on-line SPE and quantitative elution into a narrow band prior to HPLC analysis.
• Copolymeric divinylbenzene-co-n-vinylpyrrolidone beads (Oasis HLB) were used as renewable sorbent materials.

Main Results:

• The automated analytical method demonstrated high recovery percentages (>88%) and low limits of detection (0.02-0.67 ng mL(-1)).
• Coefficients of variation were consistently below 11% in the column renewable mode.
• The system achieved a significant reduction in operational costs (approximately 25-fold) compared to conventional on-line column switching systems.

Conclusions:

• The novel MSFIA-BI-LOV hyphenation offers an efficient and cost-effective solution for on-line sample processing prior to HPLC analysis.
• This approach effectively handles complex environmental and biological samples, enabling expeditious determination of pharmaceutical residues.
• The system overcomes limitations of conventional on-line SPE, minimizing band broadening and reducing operational expenses.