Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitosis and Cytokinesis02:03

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
Mitosis and Cytokinesis02:03

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Mitosis and Cytokinesis01:35

Mitosis and Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Mitosis And Cytokinesis01:35

Mitosis And Cytokinesis

In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coordinated chromosome motion emerges from mechanical coupling mediated by the physical spindle environment.

Molecular biology of the cell·2026
Same author

The Role of Transient Crosslinks in the Chromatin Search Response to DNA Damage.

International journal of molecular sciences·2025
Same author

Different relative scalings between transient forces and thermal fluctuations tune regimes of dynamic clustering.

Physical review. E·2025
Same author

The centromere bottlebrush requires a multi-microtubule attachment.

Molecular biology of the cell·2025
Same author

Centromeres are stress-induced fragile sites.

Current biology : CB·2025
Same author

Closing the loops: chromatin loop dynamics after DNA damage.

Nucleus (Austin, Tex.)·2024

Related Experiment Video

Updated: May 12, 2026

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 13, 2014

NoCut: cytokinesis in check.

Kerry Bloom1

  • 1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. kbloom@email.unc.edu

Cell
|April 18, 2006
PubMed
Summary

A new pathway called NoCut links chromosome segregation to cell division in budding yeast. This pathway uses Aurora kinase to control cytokinesis via cell polarity proteins Boi1 and Boi2.

Area of Science:

  • Cell biology
  • Molecular biology
  • Genetics

Background:

  • Cytokinesis, the process of cell division, must be precisely coordinated with the completion of chromosome segregation.
  • The regulation of this coordination is crucial for maintaining genomic stability.

Purpose of the Study:

  • To identify and characterize novel pathways that link chromosome segregation to cytokinesis in budding yeast.
  • To elucidate the molecular mechanisms governing the transition from mitosis to cell division.

Main Methods:

  • Utilized budding yeast (Saccharomyces cerevisiae) as a model organism.
  • Employed genetic and cell biological techniques to investigate protein function and pathway interactions.

Main Results:

More Related Videos

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Related Experiment Videos

Last Updated: May 12, 2026

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 13, 2014

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

  • Described a new pathway, termed NoCut, that connects chromosome segregation to cytokinesis.
  • Identified Aurora kinase (Ipl1) at the spindle midzone as a key regulator within the NoCut pathway.
  • Demonstrated that Ipl1 negatively regulates cytokinesis through its interaction with cell polarity proteins Boi1 and Boi2.

    • Conclusions:

    • The NoCut pathway provides a critical link ensuring that chromosome segregation is completed before cytokinesis initiates.
    • Aurora kinase and cell polarity proteins Boi1/Boi2 play essential roles in negatively regulating cytokinesis until proper chromosome segregation occurs.