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[Preparation and purification of polyclonal antibody against chymopapain]
Yu-lan Ding1, Tai-cheng Yang, Shu-jin Zhao
1Guangzhou General Hospital of Guangzhou Military Area, Guangzhou 510010, China.
Objective:
To prepare and purify polyclonal antibody against chymopapain, and to make a foundation for establishing an immunossay for chymopapain.
Methods:
New Zealand rabbit was immunized with chymopapain. Antiserum was purified by Protein A and analyzed by ELISA.
Results:
The titer of the antiserum obtained in this experiment by ELISA was up to 1:380000 and the purity was proved to be high by SDS-PAGE.
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