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Updated: Aug 9, 2026

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Published on: December 26, 2012
[Sodium transport and its regulation of acutely isolated alveolar type II cells]
1Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To observe the sodium current of acutely isolated alveolar type II cells and its regulation.
Methods:
After isolation the AT II cells, the whole cell sodium current were recorded in 5 hours with patch-clamp in whole-cell mode, and the effect of amiloride and terbutaline on the current was investigated.
Results:
The amiloride-sensitive current of AT II cells were recorded, which could be obviously stimulated by terbutaline.
Conclusion:
Acutely isolated AT II cells can be valuable for studying electrophysiological characteristics of sodium channels even under pathological conditions.
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