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Obscurin: a multitasking muscle giant.

Aikaterini Kontrogianni-Konstantopoulos1, Robert J Bloch

  • 1Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. akons001@umaryland.edu

Journal of Muscle Research and Cell Motility
|April 21, 2006
PubMed
Summary
This summary is machine-generated.

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Obscurin, a giant muscle protein, interacts with the sarcoplasmic reticulum and contractile apparatus. This interaction suggests a key role for obscurin in organizing muscle structure and assembly.

Area of Science:

  • Muscle biology
  • Protein biochemistry
  • Cellular organization

Background:

  • Obscurin is a giant protein in vertebrate striated muscle, similar to titin and nebulin.
  • It possesses multiple adhesion and signaling domains.
  • Unlike titin and nebulin, obscurin localizes to Z-disks and M-lines.

Purpose of the Study:

  • To investigate the role of obscurin in muscle structure.
  • To understand obscurin's interactions with other muscle proteins and organelles.

Main Methods:

  • Protein characterization
  • Immunofluorescence microscopy
  • Overexpression studies in myotubes

Main Results:

  • Obscurin binds to small ankyrin 1, an integral sarcoplasmic reticulum membrane protein.

Related Experiment Videos

  • Obscurin associates with titin and sarcomeric myosin.
  • Overexpression of obscurin's COOH-terminus affects sarcomeric myosin organization and A-band assembly.

    • Conclusions:

    • Obscurin's unique localization and binding properties suggest a role in myofibril and sarcoplasmic reticulum organization.
    • Obscurin may modulate the assembly of sarcomeric structures and the sarcoplasmic reticulum.