Related Experiment Video
Updated: Aug 9, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Investigations of the modular structure of bacterial promoters
Nora S Miroslavova1, Stephen J W Busby
1School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK.
Abstract:
Bacterial RNA polymerase holoenzyme carries different determinants that contact different promoter DNA sequence elements. These contacts are essential for the recognition of promoters prior to transcript initiation. Here, we have investigated how active promoters can be built from different combinations of elements. Our results show that the contribution of different contacts to promoter activity is critically dependent on the overall promoter context, and that certain combinations of contacts can hinder transcription initiation.
Related Concept Videos
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
Prokaryotic Gene Structure and Organization
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Operon Model

