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Related Experiment Videos

Decrease in rat internal anal pressure with the use of a topical ointment containing a killed E. coli culture

Hiroko Kido1, Hiroshi Yasukawa, Tsuyoshi Hirota

  • 1Research Laboratories, Kyoto R&D Center, Maruho Co. Ltd., 93 Awata-cho, Chudoji, Shimogyo-ku, 600-8815, Kyoto, Japan. kido_afd@mii.maruho.co.jp

International Journal of Colorectal Disease
|April 22, 2006
PubMed
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Posterisan forte (BCS+HC) and Posterisan (BCS) ointments effectively lower internal anal pressure in rats by stimulating nitric oxide production. This study clarifies their mechanism of action for potential therapeutic applications.

Area of Science:

  • Pharmacology
  • Gastroenterology
  • Physiology

Background:

  • Internal anal pressure is a key factor in anorectal function.
  • Dysregulation of internal anal pressure can lead to various anorectal disorders.
  • Topical treatments are explored for managing conditions related to elevated anal pressure.

Purpose of the Study:

  • To elucidate the mechanism by which an Escherichia coli culture suspension and hydrocortisone ointment (Posterisan forte, BCS+HC) reduces internal anal pressure.
  • To investigate the role of nitric oxide in the pressure-lowering effects of BCS+HC and BCS ointments.

Main Methods:

  • Internal anal pressure was measured in conscious rats using a water-filled balloon system.
  • Dose-dependent effects of BCS+HC ointment were assessed.

Related Experiment Videos

  • The impact of NG-nitro-L-arginine methyl ester HCl (L-NAME), a nitric oxide synthase inhibitor, was evaluated.

    • Main Results:

    • BCS+HC ointment significantly decreased internal anal pressure in a time- and dose-dependent manner.
    • Both BCS+HC and hydrocortisone-free BCS ointments demonstrated significant pressure-lowering effects compared to placebo.
    • The pressure-lowering effect of BCS+HC was significantly suppressed by L-NAME.

    Conclusions:

    • Topical application of BCS+HC and BCS ointments containing an E. coli culture suspension effectively lowers internal anal pressure in rats.
    • The findings suggest that endogenous nitric oxide production mediates the pressure-lowering effects of these ointments.
    • BCS+HC also demonstrated efficacy in reducing capsaicin-induced high internal anal pressure.