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Related Experiment Videos

Individual microflora beget unique oral microcosms.

R G Ledder1, P Gilbert, A Pluen

  • 1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester, UK.

Journal of Applied Microbiology
|April 25, 2006
PubMed
Summary
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The multiple Sorbarod device (MSD) successfully replicates individual human oral microbiotas in vitro. This advancement allows for more relevant microcosm studies by accounting for inter-individual variations in salivary composition.

Area of Science:

  • Microbiology
  • Oral Health
  • Biofilm Research

Background:

  • The human oral microbiota is complex and varies significantly between individuals.
  • Understanding these inter-individual variations is crucial for accurate oral health research.
  • Existing in vitro models often fail to capture this crucial variability.

Purpose of the Study:

  • To evaluate the efficacy of the multiple Sorbarod device (MSD) in replicating inter-individual variations of oral microbiotas.
  • To assess the MSD's capability in growing and sampling oral biofilms (BF) and released cells (perfusates, PAs).

Main Methods:

  • Two sets of triplicate MSDs were inoculated with saliva from either multiple (DV) or single (SV) volunteers.
  • Biofilms and perfusates were analyzed over 24-hour intervals using PCR-denaturing gradient gel electrophoresis (DGGE) of 16S rDNA.

Related Experiment Videos

  • Hierarchical dendrograms were constructed to analyze community relatedness and variations over time.

    • Main Results:

    • The MSD supported complex oral communities with high bacterial diversity, including anaerobic species.
    • Significant inter-individual variations in species abundance were observed in MSDs inoculated from different volunteers (DV).
    • MSDs inoculated from a single volunteer (SV) showed less variation between replicates, indicating successful model standardization.

    Conclusions:

    • The multiple Sorbarod device (MSD) can partially replicate marked inter-individual variations found in human salivary microbiotas.
    • This study demonstrates the successful in vitro reproduction of individual oral microbiotas.
    • Incorporating inter-individual variability into microcosm studies enhances their relevance and accuracy.