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Related Experiment Videos

Flow injection system based on the sandwich technique for saving expensive reagents.

A N Araujo1, J L Lima, J Alonso-Chamarro

  • 1Departamento de Química Física, Faculdade de Farmacia, Porto, Portugal.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|November 9, 1991
PubMed
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A novel sandwich technique in flow injection systems significantly reduces reagent consumption for glucose determination. This method allows for 450 tests using the same reagents as 50 batch tests, improving efficiency.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Clinical Diagnostics

Background:

  • Flow injection analysis (FIA) systems offer potential for efficient chemical analysis.
  • Reducing reagent consumption is crucial for cost-effective diagnostic assays.
  • Enzymatic methods for glucose determination are widely used in clinical settings.

Purpose of the Study:

  • To develop and evaluate a sandwich technique for flow injection analysis.
  • To assess the reagent-saving potential and applicability of the developed technique.
  • To determine glucose in serum samples using the enzymatic glucosidase/peroxidase method within the FIA system.

Main Methods:

  • Application of a sandwich technique within a flow injection system.
  • Incorporation of two reagent recirculation systems.

Related Experiment Videos

  • Enzymatic determination of glucose using glucosidase/peroxidase in serum samples.

    • Main Results:

    • Significant reduction in expensive reagent consumption compared to batch procedures (450 vs. 50 determinations per reagent amount).
    • Achieved a sampling rate of 80 determinations per hour.
    • Obtained a relative standard deviation of 0.9% for glucose determination.

    Conclusions:

    • The developed sandwich technique in flow injection systems offers substantial reagent savings.
    • The technique is applicable for accurate and efficient glucose determination in serum.
    • This approach enhances the cost-effectiveness and throughput of diagnostic assays.