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Updated: Aug 9, 2026
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
Propionibacterium acnes DNA from an explanted intraocular lens detected by polymerase chain reaction in a case of
Jui-Yang Lai1, Ko-Hua Chen, Ying-Cheng Lin
1Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, China.
Abstract:
We report a case of Propionibacterium acnes DNA from the explanted intraocular lens (IOL) a 76-year-old man with delayed-onset chronic endophthalmitis. The detection was by polymerase chain reaction (PCR). Slitlamp microscopic examination showed cells and flare in the anterior chamber, keratic precipitates on the endothelium, and multiple white plaques on both surfaces of the IOL. Propionibacterium acnes DNA was found in both the aqueous humor and the explanted IOL by PCR. Scanning electron microscopy showed diphtheroid particles, a coating of biofilm-like deposits, as well as scattered crystals on the surface of the explanted IOL and supported the possibility of P acne colonies on the IOL.
Insights
Propionibacterium acnes DNA was detected in an explanted intraocular lens (IOL) from a patient with chronic endophthalmitis. This finding supports P. acnes as a cause of delayed-onset infection after IOL surgery.
Area of Science:
- Ophthalmology
- Microbiology
- Medical Diagnostics
Background:
- Delayed-onset chronic endophthalmitis following intraocular lens (IOL) implantation is a challenging clinical entity.
- Identifying the causative pathogen is crucial for effective treatment and preventing vision loss.
Observation:
- A 76-year-old male presented with delayed-onset chronic endophthalmitis.
- Slit-lamp examination revealed anterior chamber inflammation, keratic precipitates, and white plaques on the explanted IOL.
- Scanning electron microscopy showed biofilm-like deposits and diphtheroid particles on the IOL surface.
Findings:
- Polymerase chain reaction (PCR) detected Propionibacterium acnes DNA in both the aqueous humor and the explanted IOL.
- Microscopic findings supported the presence of P. acnes colonies and biofilm formation on the IOL.
Implications:
- This case highlights the utility of PCR in diagnosing P. acnes endophthalmitis.
- The findings suggest that P. acnes can form biofilms on IOLs, contributing to chronic inflammation.
- Early detection and targeted antimicrobial therapy are essential for managing P. acnes-related endophthalmitis.
