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Published on: October 4, 2024
Quality control of PET radiopharmaceuticals using HPLC with electrochemical detection
Ryuji Nakao1, Kenji Furutuka, Masatoshi Yamaguchi
1Department of Medical Imaging, National Institute of Radiological Sciences, Chiba 263-8555, Japan. nakaor@nirs.go.jp
Nuclear Medicine and Biology
|April 25, 2006
Summary
High-performance liquid chromatography with electrochemical detection (HPLC/ECD) effectively controls positron emission tomography (PET) radiopharmaceuticals. This HPLC/ECD method offers ppb detection limits for many PET compounds, surpassing UV detection.
Area of Science:
- Analytical Chemistry
- Radiopharmaceutical Chemistry
Background:
- Quality control of radiopharmaceuticals is crucial for accurate diagnostic imaging.
- Positron emission tomography (PET) relies on radiopharmaceuticals for molecular imaging.
- Existing analytical methods may have limitations in sensitivity or applicability.
Purpose of the Study:
- To evaluate the utility of high-performance liquid chromatography with electrochemical detection (HPLC/ECD) for quality control of PET radiopharmaceuticals.
- To determine the detection limits and applicability of HPLC/ECD for various PET compounds and their precursors.
Main Methods:
- Reversed-phase chromatography with acetonitrile or phosphate buffer mobile phase.
- Electrochemical detection using a glassy carbon electrode against a Ag/AgCl reference electrode.
- Analysis of 21 different PET radiopharmaceuticals and their synthetic precursors.
Main Results:
- Successful detection of compounds and precursors for 19 out of 21 PET radiopharmaceuticals studied.
- Achieved parts per billion (ppb) detection limits for 17 compounds with electroactive functional groups.
- Demonstrated significantly better sensitivity compared to ultraviolet (UV) detection.
Conclusions:
- HPLC/ECD is a highly effective method for the quality control of PET radiopharmaceuticals.
- The method provides sensitive detection, particularly for compounds with electroactive functional groups.
- HPLC/ECD is suitable for analyzing PET radiopharmaceuticals lacking UV absorbance, such as [11C]MP4A.
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