Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vif-deficient HIV reverse transcription complexes (RTCs) are subject to structural changes and mutation of

J M Carr1, A J Davis, C Coolen

  • 1Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia. jill.carr@imvs.sa.gov.au

Virology
|April 25, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enzyme super-dosage mitigates growth impairment in broilers fed high levels of <i>Chlorella vulgaris</i> and improves meat fatty acid composition.

British poultry science·2026
Same author

The High-Energy Ion Telescope (HIT) for the Interstellar Mapping And Acceleration Probe (IMAP) Mission.

Space science reviews·2026
Same author

High inclusion of <i>Chlorella vulgaris</i> in broiler diets improves feed efficiency and liver n‑3 fatty acid profile: effects of extrusion and enzyme super-dosing.

British poultry science·2026
Same author

High dietary inclusion of Chlorella vulgaris with extrusion or enzyme superdosing: Effects on broiler performance, welfare, and meat quality.

Poultry science·2025
Same author

First report of a Sporidesmin Toxicity (Facial Eczema) outbreak in a South African dairy herd.

Journal of the South African Veterinary Association·2025
Same author

Reliable long-term individual variation in wild chimpanzee technological efficiency.

Nature human behaviour·2024

HIV-infected cells utilize reverse transcription complexes (RTCs) for viral DNA synthesis. Defective Vif protein in HIV leads to abnormal RTCs with increased mutations in reverse transcription products within newly infected cells.

Area of Science:

  • Virology
  • Molecular Biology
  • HIV Research

Background:

  • Reverse transcription (RTn) in HIV occurs within a nucleoprotein complex called the reverse transcription complex (RTC).
  • RTCs contain reverse transcriptase (RT) and integrase (IN) and exhibit heterogeneity in size and density.
  • The Vif protein plays a role in HIV replication, particularly in non-permissive cells.

Purpose of the Study:

  • To investigate the characteristics of RTCs in HIV infections, focusing on the impact of Vif deficiency.
  • To analyze the sedimentation properties and mutation levels of RTCs produced by Vif-deficient (Deltavif) HIV.
  • To understand the relationship between RTC structure, Vif function, and APOBEC3G-mediated mutagenesis.

Main Methods:

  • Analysis of RTCs using sucrose velocity and equilibrium gradients.

Related Experiment Videos

  • Comparison of RTCs from wild-type (WT) and Deltavif HIV produced in permissive and non-permissive cells.
  • Assessment of reverse transcription products and mutation levels within RTCs.

    • Main Results:

    • RTCs from Deltavif HIV produced in non-permissive cells showed altered sedimentation, with fewer major RTC forms and more rapidly sedimenting RTn products.
    • APOBEC3G did not co-sediment with RTCs, but Deltavif RTCs exhibited elevated mutations in RTn products.
    • The most mutated transcripts were found within rapidly sedimenting RTCs.

    Conclusions:

    • HIV lacking functional Vif, when produced in non-permissive cells, forms abnormal RTCs.
    • These abnormal RTCs are associated with increased mutation of reverse transcription products.
    • The findings highlight the role of Vif in regulating RTC formation and preventing excessive mutagenesis during HIV replication.