Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ovine cord blood accommodates multipotent mesenchymal progenitor cells.

Marcus Jäger1, Radu Bachmann, Axel Scharfstädt

  • 1Department of Orthopaedics, Heinrich-Heine University Hospital Duesseldorf D-40225 Duesseldorf Germany. Jaeger@med.uni-duesseldorf.de

In Vivo (Athens, Greece)
|April 26, 2006
PubMed
Summary

Ovine cord blood stem cells (S-USSCs) can be isolated and expanded in vitro. These mesenchymal stem cells successfully differentiated into osteoblasts, chondroblasts, and adipoblasts, demonstrating their multipotency for potential therapeutic applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ASO Visual Abstract: Correlations between the Histological Growth Patterns of Peritoneal and Liver Metastases from Colorectal Cancer.

Annals of surgical oncology·2026
Same author

Correlations Between the Histologic Growth Patterns of Peritoneal and Liver Metastases From Colorectal Cancer.

Annals of surgical oncology·2026
Same author

Intraoperative Bioactivation of Bone Substitutes Using a Surgical Suction Handle: A Prospective Clinical Pilot Study.

Journal of functional biomaterials·2026
Same author

Three-Dimensional Visualization and Proteomic Analysis of Human Cardiac Transthyretin Amyloidosis Tissue Reveals Microangiopathy and Capillary Occlusion.

Journal of the American Heart Association·2026
Same author

Penetrating Abdominal Injuries: A 10-Year Retrospective Analysis of 49 Patients at a German Trauma Center.

Medical science monitor : international medical journal of experimental and clinical research·2026
Same author

A New Subpopulation of Extracellular Vesicles Harvested from Osteogenically Induced Mesenchymal Stromal Cells of Surgical Site-Released Tissue.

Biomolecules·2026

Area of Science:

  • Stem cell biology
  • Mesenchymal stem cell research
  • Comparative medicine

Background:

  • Mesenchymal stem cells from umbilical cord blood exhibit multipotency.
  • Ovine models are used for xenotransplantation studies.
  • Differentiation potential of ovine cord blood stem cells is largely unexplored.

Purpose of the Study:

  • To isolate and characterize ovine cord blood stem cells (S-USSCs).
  • To investigate the in vitro differentiation capacity of S-USSCs into osteogenic, chondrogenic, and adipogenic lineages.
  • To confirm lineage-specific differentiation using morphological and immunocytochemical markers.

Main Methods:

  • Isolation of mononuclear cells from ovine placentomes via density gradient centrifugation.
  • In vitro expansion and lineage-specific stimulation of S-USSCs for 20 days.

Related Experiment Videos

  • Morphological characterization (light and transmission electron microscopy) and immunocytochemistry for lineage-specific markers (PPAR, OP, Collagen II, COMP).

    • Main Results:

    • S-USSCs were successfully isolated and expanded in vitro.
    • Osteogenic stimulation resulted in bone nodule formation.
    • Adipogenic stimulation led to lipid droplet development, and chondrogenic stimulation produced chondroblast-like cells.
    • Immunocytochemical analysis confirmed the presence of lineage-specific markers.

    Conclusions:

    • Ovine mesenchymal stem cells (S-USSCs) can be isolated from umbilical cord blood and expanded.
    • S-USSCs demonstrate multipotency, differentiating into osteoblasts, chondroblasts, and adipoblasts in vitro.
    • These findings validate the potential of ovine cord blood stem cells for regenerative medicine research.