Related Experiment Video
Updated: Aug 9, 2026
09:53
Gene Editing of Primary Rhesus Macaque B Cells
Published on: February 10, 2023
Cloning of rhesus monkey LILRs
I I Slukvin1, R L Grendell, D S Rao
1Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI, USA. islukvin@wisc.edu
Tissue Antigens
|April 26, 2006
Summary
Researchers identified rhesus monkey Leukocyte Ig-like receptors (LILRs), crucial for immune regulation. This discovery aids studying LILR functions in non-human primates, advancing immune system research.
Area of Science:
- Immunology
- Molecular Biology
- Primate Research
Background:
- Leukocyte Ig-like receptors (LILRs) are key immune regulators expressed on lymphoid and myeloid cells.
- LILRs possess both inhibitory and activating functions, influencing immune responses.
- Understanding LILR diversity is crucial for dissecting immune system mechanisms.
Purpose of the Study:
- To identify and characterize Leukocyte Ig-like receptors (LILRs) in rhesus monkeys.
- To explore the structural and functional diversity of rhesus LILRs.
- To establish a non-human primate model for LILR functional studies.
Main Methods:
- Screening of rhesus monkey spleen and decidua cDNA libraries.
- RT-PCR cloning techniques.
- Sequence analysis to determine structural and functional motifs.
Main Results:
- Identification of eight distinct full-length rhesus monkey LILR clones.
- Observed structural diversity including inhibitory motifs, truncated tails, and soluble forms.
- Rhesus LILR diversity mirrors that found in human LILRs.
Conclusions:
- The characterization of rhesus LILRs provides a valuable resource for immunological studies.
- Rhesus monkeys serve as a relevant model for investigating LILR roles in immune regulation.
- Further research can explore LILR interactions with non-classical MHC class I molecules in primates.
Related Concept Videos
01:27Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
01:08Cloning of Dolly the Sheep
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual, the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...
01:14Introduction to Nuclear Reprogramming
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...