Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A reverse-phase HPLC method for cAMP phosphodiesterase activity.

G Spoto1, E Whitehead, A Ferraro

  • 1Institute of Biochemical Sciences, University of Chieti G. D'Annunzio, Italy.

Analytical Biochemistry
|August 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Projectile deformation on human bone and on a polyurethane simulant.

International journal of legal medicine·2025
Same author

Morphological and functional analysis of cryopreserved human sperm: comparison of different freezing protocols.

Cryo letters·2025
Same author

The influence of a skin simulant on bullet trajectory deflection in ballistic gelatine.

Forensic science international·2025
Same author

A narrative review on diagnosis and treatment of ifosfamide-induced encephalopathy, the perspective of a EURACAN reference center for sarcomas.

Frontiers in pharmacology·2025
Same author

Radiological evaluation of response in patients with locally advanced/metastatic soft tissue sarcoma treated with trabectedin.

Frontiers in pharmacology·2024
Same author

Extracorporeal bullet trajectory determination from scanned phantoms with bullet defects.

Legal medicine (Tokyo, Japan)·2024
Same journal

The Long Run: A Tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the Mechanism of He Xue Ming Mu Tablets on Non-Proliferative Diabetic Retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
Same journal

A PCR-based assay for distinguishing between 293, 293T, and 293E cell lines.

Analytical biochemistry·2026
See all related articles

A new reverse-phase HPLC method rapidly measures cAMP phosphodiesterase activity. This sensitive and reproducible technique accurately quantifies nucleotides in biological samples for kinetic studies.

Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Cyclic adenosine monophosphate (cAMP) phosphodiesterase (PDE) is a crucial enzyme regulating intracellular signaling.
  • Accurate measurement of PDE activity is essential for understanding cellular processes and drug discovery.

Purpose of the Study:

  • To develop a simple, fast, and sensitive method for measuring cAMP phosphodiesterase activity.
  • To enable accurate quantitation of substrate and product for kinetic analyses.

Main Methods:

  • Development of a reverse-phase High-Performance Liquid Chromatography (HPLC) method.
  • Quantitation of adenosine monophosphate (AMP) as a product and cAMP as a substrate.

Main Results:

  • The method provides results in under 10 minutes.

Related Experiment Videos

  • Achieved high sensitivity with a limit of 1x10(-11) mol AMP.
  • Demonstrated accurate nucleotide evaluation, unequivocal product analysis, and system reproducibility.

    • Conclusions:

    • The developed HPLC method is suitable for evaluating cAMP phosphodiesterase activity.
    • The method is applicable to biological materials at various purification levels.
    • This technique facilitates efficient kinetic studies of cAMP phosphodiesterase.