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Updated: Aug 9, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
[The kinetic characteristics of lipids of animal tissues in autooxidation reactions]
Abstract:
The results of long-time research of antioxidative activity (AOA) of lipids from tissues of different species and lines of laboratory rodents are generalized. The classification of lipids according to their ability to inhibit the thermal autooxidation of methyl oleate is proposed. The participation of lipids in low-temperature autooxidation reactions at the initiation and chain propagation stages was proved by means of the model proposed. In addition to the lipid antioxidative activity, the initial quantity of peroxides in lipids due to the extent of their unsaturation and lipid antiperoxide activity are proposed for the estimation of the kinetic characteristics of lipids. The dependence of effects on the rate of radical initiation in the system is shown to be caused by the influence of physicochemical properties of lipids on the interrelation coordination and balance of biochemical functions in biological objects differing in the intensity of oxidation processes.
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