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Construction and characterization of Bacillus subtilis deletion mutants lacking the prophage 2-trnS region
Genki Akanuma1, Chiemi Habu, Yousuke Natori
1Laboratory of Molecular Genetics and Frontier Project Life's Adaptation Strategies to Environmental Changes, Department of Life Science, College of Science, Rikkyo University, Tokyo, Japan.
Abstract:
During development of a novel method for constructing a series of deletions in Bacillus subtilis using an isogenic set of gene-disrupted mutants created by integration of pMutin, deletion of the trnS operon, consisting of seven tRNA genes, was found to affect cell growth, development of competence and spore formation. A suppressor (sts1) of the DeltatrnS mutant was isolated, sequenced and found to have undergone a single base change, CAG to GAG, in the first anticodon of tRNA(Leu), in the trnB operon.
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