Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane-associated transcripts in Arabidopsis; their isolation and characterization by DNA microarray analysis and

Mark de Jong1, Bas van Breukelen, Floyd R Wittink

  • 1Molecular Genetics Group, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

The Plant Journal : for Cell and Molecular Biology
|April 28, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Lettuce Expression Browser: from lab to LEB.

The Plant journal : for cell and molecular biology·2026
Same author

A phosphorelay circuit drives extracellular alkalinization in receptor kinase-mediated immune and cell-wall damage signaling.

Molecular cell·2026
Same author

Publisher Correction: Motif-based substrate mapping of the receptor-like cytoplasmic kinase BIK1 reveals novel components and regulatory nodes of plant immunity.

Nature plants·2026
Same author

Motif-based substrate mapping of the receptor-like cytoplasmic kinase BIK1 reveals novel components and regulatory nodes of plant immunity.

Nature plants·2026
Same author

Pangenome graph analysis reveals evolution of resistance breaking in spinach downy mildew.

PLoS biology·2026
Same author

Genetic suppressors of the growth-immunity trade-off in the Arabidopsis salicylic acid-accumulating dmr6 dlo1 mutant.

The Plant journal : for cell and molecular biology·2025

Researchers developed a new method to isolate mRNA from membrane-bound polysomes in Arabidopsis. This technique efficiently identifies potential membrane-associated and secreted proteins, advancing our understanding of plant cell biology.

Area of Science:

  • Plant Molecular Biology
  • Cellular Biology
  • Proteomics

Background:

  • Integral membrane and secreted proteins are crucial for cellular functions, but their localization is often unconfirmed in Arabidopsis.
  • Current prediction algorithms for transmembrane domains and signal peptides have limitations in accuracy and comprehensiveness.
  • Identifying these proteins is vital for understanding plant cellular processes.

Purpose of the Study:

  • To develop and validate an efficient method for isolating mRNA from membrane-bound polysomes.
  • To enrich for transcripts encoding membrane-associated and secreted proteins genome-wide.
  • To identify novel, uncharacterized secreted or membrane-associated proteins in Arabidopsis.

Main Methods:

  • Isolation of mRNA from membrane-bound polysomes.

Related Experiment Videos

  • Validation using DNA microarray analysis.
  • Assessment of reproducibility through Pearson correlations of transcript levels from replicate isolations.

    • Main Results:

    • The developed method is highly reproducible across multiple replicates.
    • Significant enrichment of transcripts encoding proteins with predicted transmembrane domains and signal peptides was observed.
    • 301 transcripts in the membrane-bound polysomal fraction were annotated as 'cellular component unknown', suggesting novel proteins.

    Conclusions:

    • The isolation of membrane-bound polysomal mRNA is an effective strategy for identifying membrane-associated and secreted proteins.
    • This method facilitates the genome-wide discovery of previously unrecognized proteins in Arabidopsis.
    • The findings contribute to a more comprehensive understanding of the Arabidopsis proteome and cellular localization.