Related Experiment Video
Updated: Aug 2, 2026

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
Published on: January 30, 2016
Automated determination of the center of rotation in tomography data
Tilman Donath1, Felix Beckmann, Andreas Schreyer
1GKSS-Research Center, Geesthacht, Germany. tilman.donath@gkss.de
Abstract:
Tomographic reconstruction requires precise knowledge of the position of the center of rotation in the sinogram data; otherwise, artifacts are introduced into the reconstruction. In parallel-beam microtomography, where resolution in the 1 microm range is reached, the center of rotation is often only known with insufficient accuracy. We present three image metrics for the scoring of tomographic reconstructions and an iterative procedure for the determination of the position of the optimum center of rotation. The metrics are applied to model systems as well as to microtomography data from a synchrotron radiation source. The center of rotation is determined using the image metrics and compared with the results obtained by the center-of-mass method and by image registration. It is found that the image metrics make it possible to determine the axis position reliably at well below the resolution of one detector bin in an automated procedure.
Related Concept Videos
Finding the Center of Gravity
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Computed Tomography
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Centroid for the Paraboloid of Revolution
The centroid for the paraboloid of revolution is the point where all the mass of the paraboloid is concentrated. This centroid is important for engineering applications, as it determines how forces are...
Relative Motion Analysis using Rotating Axes-Problem Solving
Here, in order to determine the magnitude of velocity and acceleration for point...
Applications of Integration to Find Centers of Mass

