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Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regions

I x Sevilla1, S V Singh, J M Garrido

  • 1Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER), Berreaga 1, 48160 Derio, Bizkaia, Spain.

Revue Scientifique Et Technique (International Office of Epizootics)
|April 29, 2006
PubMed

Insights

Genetic differences in Mycobacterium avium subsp. paratuberculosis (Map) were found across Spain, India, and the USA. These Map typing results can aid in tracing paratuberculosis outbreaks and understanding its epidemiology.

Area of Science:

  • Veterinary Microbiology
  • Molecular Epidemiology
  • Bacterial Genetics

Background:

  • Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (Map), affects livestock globally.
  • Understanding the genetic diversity of Map is crucial for effective disease control and epidemiological tracing.

Purpose of the Study:

  • To investigate the genetic variations among Map isolates from different hosts and geographical regions.
  • To assess the utility of IS1311 polymerase chain reaction-restriction endonuclease analysis (PCR-REA) for Map typing.

Main Methods:

  • IS1311 polymerase chain reaction-restriction endonuclease analysis (PCR-REA) was employed.
  • Thirty-eight Map isolates from cattle, sheep, goats, and bison across Spain, India, and the USA were analyzed.

Main Results:

  • Distinct genetic profiles (C, S, and B types) were identified among Map isolates.
  • Isolate types correlated with geographic origin and host species, indicating host and geographic factors influence Map genetics.
  • Specific types were prevalent in certain regions: C and S types in Spain, B type in India and the USA.

Conclusions:

  • Significant genetic differences exist within Mycobacterium avium subsp. paratuberculosis isolates.
  • Geographic location and host animal are important factors shaping Map genetic diversity.
  • The IS1311 PCR-REA method is a valuable tool for epidemiological tracing of paratuberculosis.

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