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Updated: Aug 9, 2026

Using Solution NMR to Characterize Biomolecular Condensates Under Biphasic Conditions
Published on: April 17, 2026
Biexponential fitting of diffusion-ordered NMR data: practicalities and limitations
Mathias Nilsson1, Mark A Connell, Adrian L Davis
1School of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, UK. mathias.nilsson@manchester.ac.uk
Abstract:
High resolution diffusion-ordered NMR spectroscopy (HR-DOSY) generally uses monoexponential fitting of the diffusional attenuation of pulsed-field gradient stimulated echo spectra and can, thus, only give proper separation of signals in the diffusion domain where the individual peaks are well-resolved in the spectral domain. In principle, it should be possible to resolve the decays of coincident spectral peaks by multiexponential fitting, but the well-known fundamental difficulties in such a separation are exacerbated by instrumental distortions of the signal decay. The limitations imposed on biexponential fitting by finite signal-to-noise ratio and by the distortion of the theoretical form of the signal attenuation as a result of pulsed-field gradient nonuniformity are explored, and the improvement in DOSY spectra produced using biexponential fitting afforded by compensating for the latter are illustrated.
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