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Updated: Aug 9, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Study on capillary electrophoresis-amperometric detection profiles of different parts of Morus alba L
Qingcui Chu1, Miao Lin, Xiuhui Tian
1Department of Chemistry, East China Normal University, Shanghai 200062, China.
Abstract:
A high-performance capillary electrophoresis with amperometric detection (CE-AD) method has been developed for the determination of the pharmacologically active ingredients in different parts of Morus alba L. after a relatively simple extraction procedure. This method was also used in the comparison of bioactive constituent difference in the five parts, based on their electropherograms or characteristic "CE-AD profiles". The effects of several factors such as the acidity and concentration of running buffer, separation voltage, applied potential and injection time were investigated to find the optimum conditions. Method detection limits (S/N = 3) ranged from 1.5 x 10(-7) to 1.4 x 10(-8)g/mL for all 10 analytes, and the assay results were satisfactory.
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