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Updated: Jul 28, 2026

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
Published on: April 2, 2012
[Temperature-sensitive clones of herpes simplex virus type 1 from laboratory and clinical strains. I. Cloning and
B Litwińska1, W Sadowski, M Kańtoch
1Zakład Wirusologii PZH w Warszawie.
Abstract:
Two HSV-1 strains were used in the study: McIntyre laboratory strain and "eye" strain isolated from a patient. Temperature-sensitive clone of HSV-1 was isolated from McIntyre strain as a consequence of virus replication carried out at lowered temperature (28 degrees C). Temperature-resistant clones were obtained from both strains through passages at 39 degrees C and through heating for four times at 45 degrees C. Pathogenic properties of the temperature clones obtained were determined in inbred mice Balb/c and CFw/Pzh. A loss of pathogenicity for mice of temperature-sensitive clone and an increase of pathogenicity of temperature-resistant clones were noted as compared to parental strains. It was found that an introduction of temperature-sensitive clone, with lowered virulence immunizes against highly virulent temperature-resistant clone.
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