Sperm proteins that serve as receptors for the zona pellucida and their post-testicular modification

The search for molecules on the surface of mammalian sperm that are responsible for the binding of sperm to the zona pellucida has led to the identification of a number of different surface proteins. Different experimental approaches have been used to identify these proteins and the strength of the evidence for their putative role in zona binding is therefore quite variable. In this paper we have discussed some of the approaches that are used to identify cell adhesion molecules and criteria that might be applied in future research. Further research will be required to answer questions as to whether multiple surface antigens are involved in zona binding and if the sperm receptors for the zona are conserved among species. Both of these results would be expected based on what is known about cell adhesion in other systems. We have also discussed the modifications that occur to surface proteins after the sperm leave the testis and how these modifications can potentially activate or increase the efficiency of the function of a protein in zona binding.