Related Experiment Video
Updated: Aug 9, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Escherichia coli engineered to produce eicosapentaenoic acid becomes resistant against oxidative damages
Takanori Nishida1, Yoshitake Orikasa, Yukiya Ito
1Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan.
Abstract:
The colony-forming ability of Escherichia coli genetically engineered to produce eicosapentaenoic acid (EPA) grown in 3mM hydrogen peroxide (H(2)O(2)) was similar to that of untreated cells. It was rapidly lost in the absence of EPA. H(2)O(2)-induced protein carbonylation was enhanced in cells lacking EPA. The fatty acid composition of the transformants was unaffected by H(2)O(2) treatment, but the amount of fatty acids decreased in cultures of cells lacking EPA and increased in cultures of cells producing EPA, suggesting that cellular EPA is stable in the presence of H(2)O(2) in vivo and may protect cells directly against oxidative damage. We discuss the possible role of EPA in partially blocking the penetration of H(2)O(2) into cells through membranes containing EPA.
Related Concept Videos
Stringent Response in E. coli
Bacterial Gastroenteritis
Clinical Significance of Antibiotic Resistance
Mechanism of Antibiotic Resistance in MRSA
Evolution of New Traits in Microbes
